Dados do Resumo

Título

CORRELATION BETWEEN SERUM CYTOKINE LEVELS AND CLINICAL CHARACTERISTICS IN PATIENTS WITH COLORECTAL CANCER TREATED AT A TERTIARY HEALTHCARE UNIT IN AMAZONAS

Introdução

Inflammation plays different roles in carcinogenesis and cancer progression. The tumor environment is capable of mediating the interaction between cancer cells and the host. The correlation between serum cytokine levels and clinical characteristics in patients with colorectal cancer is essential to understanding the relationship between inflammatory markers and disease progression.

Objetivo

To investigate the correlation between serum cytokine levels and clinical characteristics of patients with colorectal cancer treated at a tertiary healthcare unit in Amazonas.

Métodos

This cross-sectional, descriptive and analytical study is part of a larger project entitled “Analysis of the molecular and clinical spectrum of colorectal cancer: from epidemiology and quality of life to genetics and omics analysis”. Approved by FCecon's Ethics Committee (CAEE 52343821.2.0000.0004, opinion 5.180.654), it included patients with colorectal adenocarcinoma, of both sexes, ≥18 years old, without previous treatment and with a signed Informed Consent Form. 10 mL of peripheral blood was collected in a tube with EDTA, centrifuged to isolate the plasma and stored at -80°C until analysis. Cytokines IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNF were measured by flow cytometry using commercial kits (BDTM Human Inflammatory Cytokines Kit and BD TM Human Cytokine Th1/Th2/Th17 Kit, BD Biosciences, San Diego, CA USA).

Resultados

The study included 36 patients with colorectal adenocarcinoma, predominantly women (55.6%) with a mean age of 61.6 years (SD=13.2). Most of the participants were brown (72.2%) and from Amazonas (61.1%). SOE adenocarcinoma (without other specifications) was the most common type (88.89%). The histological grade revealed 30.6% with well-differentiated cancer, 63.9% moderately differentiated and 5.6% poorly differentiated. The predominant lesions were in the sigmoid colon (44.4%), on the left side of the colon (75%). In terms of staging, 11.1% were stage I, 13.9% stage II, 38.9% stage III and 35.1% stage IV. Metastasis was observed in 36.1% of cases, and seven (19.4%) patients died. There were no statistically significant differences between serum cytokine levels (IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF) and tumor location, staging or metastasis.

Conclusões

The study found no significant differences between serum levels of cytokines (IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF) and clinical characteristics of colorectal cancer, such as tumor location, staging or metastasis. However, the literature suggests that alterations in these cytokines may be associated with the prognosis of the disease. The lack of significance may result from factors such as sample size. Further research is necessary to confirm these associations and their clinical impact.

Financiador do resumo

FAPEAM – Programa PAIC 23/24; EDITAL N. 002/2021 - PROGRAMA AMAZÔNIDAS; RESOLUÇÃO N. 002/2008, 007/2018 e 005/2019 - PRÓ-ESTADO REDE GENÔMICA DE VIGILÂNCIA EM SAÚDE: OTIMIZAÇÃO DA ASSISTÊNCIA E PESQUISA NO ESTADO DO AMAZONAS - REGESAM

Palavras Chave

Câncer Colorretal; Citocinas; Amazonas